rabbit anti trpv2  (Alomone Labs)

Journal: Frontiers in Immunology

Article Title: Discovering single cannabidiol or synergistic antitumor effects of cannabidiol and cytokine-induced killer cells on non-small cell lung cancer cells

doi: 10.3389/fimmu.2024.1268652

Figure Lengend Snippet: Characterization of intrinsic alternation in the CIK cells in the CBD-inducing culture by flow cytometric analysis. (A) CIK cells were exposed to either CBD or CBD combined with the TRPV2 channel antagonist tranilast at 37°C for 1 minute. Dead cells were gated and excluded by Hoechst 33258 for Fluo-4 AM expression. (B) The phosphorylation levels of Erk1/2 in CIK cells were determined by flow cytometry. Dead cells were gated and excluded by Zombie Aqua™ dye for intracellular expression. The rabbit of isotype controls is indicated. The percentage of FITC anti-ERK1/2 Phospho (Thr202/Tyr204) was analyzed using the Flowjo V10 software. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. CIK combined with DMSO control. All data are shown as the mean ± SD, representative of four independent experiments. Statistical analysis was performed using a two-way ANOVA followed by Dunnett’s multiple comparisons test by GraphPad Prism software version 9.0.0. CIK cells were derived from 4 donors.

Article Snippet: In order to detect TRPV2 expression, the anti-TRPV2 polyclonal rabbit antibody (EpigenTek, Farmingdale, NY, U.S.A) was diluted 1:200, and the secondary antibody Donkey Anti-Rabbit IgG NorthernLightsTM NL557-conjugated antibody (R&D Systems, Inc., Minneapolis, Minnesota, U.S.A.) was diluted 1:500 in dilution buffer (1% BSA, 0.3% Triton in PBS).The slide was finally stained with a diluted DAPI solution.

Techniques: Expressing, Phospho-proteomics, Flow Cytometry, Software, Control, Derivative Assay

Journal: EMBO Reports

Article Title: The mechanosensitive TRPV2 calcium channel promotes human melanoma invasiveness and metastatic potential

doi: 10.15252/embr.202255069

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti‐TRPV2 (Prestige Antibodies® Powered by Atlas Antibodies) , Sigma‐Aldrich , Cat # HPA044993, RRID:AB_10960889.

Techniques: Mouse Assay, Microarray, Recombinant, shRNA, Control, Sequencing, In Situ, Activity Assay, Clinical Proteomics, Software, Imaging, Fluorescence